This gene is the cellular homolog of viral raf gene (v-raf).The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly.Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2.Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Target	RAF1
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	ELISA, WB, IHC, FCM
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Purified recombinant fragment of human RAF1 expressed in E. coli.
Purification	Purified antibody
Isotype	IgG1
Conjugation	Unconjugated
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	73 kDa
Swiss Prot	P04049
GeneID	5894
Gene Symbol	RAF1
OMIM	164760
HGNC	9829
Ensembl	ENSG00000132155
Concentration	1.0 mg/ml
Buffer	PBS with 0.05% sodium azide and 0.5% protein stabilizer.
UNSPSC Code	12352203
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.