Proteinase K is classified as a non-specific serine protease. It is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Applications: ? Isolation of high molecular weight DNA ? Isolation of plasmid and genomic DNA ? Isolation of RNA ? Inactivation of RNase and DNase activities Advantages: ? Ready to use, provided in liquid form containing 40% glycerol. ? Smaller contamination risk with liquid form. ? Can be stored at -20°C still having the enzyme in liquid form. ? Excellent stability Notes: ?Proteinase K is not inactivated by metal-chelating agents like EDTA, by thiol-reactive reagents (such as monoiodoacetic acid, p-chloromercuribenzoate) or by specific trypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme. ?Proteinase K is stable over a wide pH range: 4-12.5. ?Raising the temperature of the reaction from 37°C to 50°-65°C can increase t