This CLIA kit applies to the in vitro quantitative determination of OPN concentrations in serum, plasma and other biological fluids.This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to OPN. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OPN and Avidin-Horseradish Peroxidase (HRP) conjugate is added