Recombinant full-length human HSP70 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
Size
50 μg
Concentration
0.2 μg/μL
Applications
Western Blot. HSP70 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. HSP70 Protein is suitable for use in Western Blot and ATPase Assay. Expect a band approximately ~96kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Other Names
HSPA1A, HSP72, HSPA1, HSPA1B, HSP70-1, Heat shock 70 kDa protein 1A/1B , Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP70.1/HSP70.2