Non-viable cell pellet was generated from cell line WM3000. WM3000 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3000 cells are from same patient as WM 2032. These cells produce xenograft tumors when injected into immunocompromised mice.
Size
2 mg
Concentration
2.0 million cells
Applications
Frozen non-viable cell pellets can be used for in vitro experiments such as Western blotting and other immunoassays, genomic DNA isolation, STR profiling, and RNA isolation.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft