3D Cell Culture HTS Cell Viability Complete Assay Kit
Description
Three dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion. 3D cell cultures improve the function, differentiation and viability of cells and recapitulate in vivo microenvironment compared to conventional 2D cell cultures. 3D matrices provide a physiologically relevant screening platform, by mimicking the in vivo responses, for many cell types including cancer and stem cells in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. Quantification of number of viable cells is an indispensable tool in in vitro screening in these studies. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetr
Size
100 assays
Concentration
n/a
Applications
? Matrix and spheroid dissociations from 3D cell culture for cell growth assessment? Measurement of cell viability in response to growth factors, cytokines, mitogens and nutrients? Analysis of cytotoxic/cytostatic compounds that affect cell growth and spheroid formation, such as anticancer drugs, toxic agents and other pharmaceuticals