Immunogen sequence: K25-Q223. Specificity: Natural and recombinant human ICAM-2. There is no detectable cross-reactivity with other relevant proteins.
Size
1 kit
Concentration
n/a
Applications
ELISA. Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human ICAM-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, tissue homogenates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human ICAM-2 standard solution and each sample be measured in duplicate.