Total RNA was prepared from cell line WM51. WM51 is a metastatic human melanoma cell line that was established from a metastatic tissue site of a male patient. These cells display a mixed morphology and require sub-confluent culture conditions. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses wild type N-RAS, c-KIT, and CDK4 genes. WM51 cells produce xenograft tumors when injected into immunocompromised mice.
Size
25 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft