Monkey IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Monkey IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Monkey Serum and anti-Monkey IgM (μ chain specific). No reaction was observed against anti-Monkey IgG F(c). Some light chain cross reactivity will occur with anti-Monkey IgG.
Size
250 μg
Concentration
1.0 mg/mL
Applications
ELISA, Immunohistochemistry, Western Blot. Monkey IgM whole molecule can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments. Monkey IgM whole molecule is stable at 4° C prior to restoration. It is recommended to aliquot restored Monkey IgM whole molecule after adding equal parts glycerol for storage at -20° C for extended storage and to prevent repeated freeze-thaw cycles.