Molecular weight marker for accurate protein sizing on SDS-polyacrylamide gels and Western blots. Electrophoresis of the marker on 13-18% Tris-glycine SDS-polyacrylamide gel resolves into 8 individual bands. The bands may not resolve if the gel % is too high or too low. Additional bands may be observed if there is DTT oxidation in the storage buffer. This protein marker is intended for use with SDS-PAGE and should not be used for native electrophoresis or native gel. This marker is denatured and contains SDS in the storage buffer. SDS in the transfer buffer will disrupt the hydrophobic interactions between the membrane and the protein. There should be no more than 0.02% SDS in the transfer buffer. Alcohol can be used to enhance binding. Effective size range: 10 kDa - 70 kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa, 70 kDa. The 26 kDa, 18 kDa and 10 kDa bands are at double intensity to make the size approximation of the protein of interest easier.