The ES-2 cell line was established from a surgical tumor specimen taken from a 47 year old black woman. This cell line was isolated from a poorly differentiated ovarian clear cell carcinoma.Initially, the cells were grown in soft agar.The cells exhibit low to moderate resistance to a number of chemotherapeutic agents including doxorubicin, cisplatin, carmustine, etoposide and cyanomorpholinodoxorubicin (MRA-CN). ES-2 cells express low levels of P glycoprotein.