ELISA, Immunohistochemistry, IF Microscopy, Immunoprecipitation, Western Blot. Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IP, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
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Description
Anti-IDO1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.
Immunogen
Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
Batista CAE., Juhasz C., Muzik O., Kupsky WJ., Barger G., Chugani HT., Mittal S., Sood S., Chakraborty PK., Chugani DC., (2009) Imaging Correlates of Differential Expression of Indoleamine 2,3-Dioxygenase in Human Brain Tumors. Mol Imaging Biol 11:460Y466. DOI: 10.1007/s11307-009-0225-0.Meyer KC., Klatte JE., Dinh H.V., Harries M.J., Reithmayer K., Meyer W., Sinclair R., Paus R. (2008) Evidence that the bulge region is a site of relative immune privilege in human hair follicles. British Journal of Dermatology 159, pp1077-1085.Lingqiao Zhu, Fang Ji, Yuan Wang, Yi Zhang, Qiang Liu, Jingwu Z. Zhang, Kouji Matsushima, Qi Cao and Yanyun Zhang. (2006) Synovial Autoreactive T Cells in Rheumatoid Arthritis Resist IDO-Mediated Inhibition. J. Immunol.177;8226-8233.