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| Antigenic Specificity | Dopamine Transporter Phospho T53, internal |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | rat |
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| Isotype | n/a |
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| Format | affinity purified, no preservative |
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| Size | 100 μL |
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| Concentration | Sufficient for 10 mini blots (total final volume 100 ml at 1:1000 dilution) |
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| Applications | ELISA, Western Blot. Anti-Phospho-Thr53 Dopamine Transporter Antibody is stored with 100 μg per ml BSA. It is suitable for Western Blots and is specific for the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel. Immunolabeling of the DAT band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. Specific conditions for reactivity should be optimized by the end user. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-Phospho-Thr53 Dopamine Transporter Antibody was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
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| Immunogen | Anti-Phospho-Thr53 Dopamine Transporter Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of rat DAT conjugated to KLH. |
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| Other Names | Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3 |
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| Gene, Accession # | SLC6A3, Gene ID: 24898, NCBI: NP_036826.1, UniProt: P23977 |
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| Catalog # | 600-401-X03 |
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| Price | |
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| Order / More Info | Dopamine Transporter Phospho T53, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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