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| Antigenic Specificity | BUBR1 Kinase Kinase, N-terminus |
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| Clone | 8G1 |
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| Host Species | Mouse |
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| Reactive Species | human |
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| Isotype | IgG1 |
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| Format | Protein A purified |
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| Size | 100 μg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, IF Microscopy, Immunoprecipitation, Western Blot. This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This Protein A purified antibody is directed against human BUBR1 Kinase protein. The product was purified from tissue culture supernatant by chromatography. This antibody has only been tested on human cells. Reactivity against homologues from other sources is not known. |
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| Immunogen | This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein. |
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| Other Names | mouse anti-BUBR1 antibody, Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody |
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| Gene, Accession # | BUBR1, Gene ID: 701, NCBI: O60566.3, UniProt: O60566 |
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| Catalog # | 200-301-902 |
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| Price | |
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| Order / More Info | BUBR1 Kinase Kinase, N-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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