A macrolide-type antibiotic that inhibits pyruvate carboxylases from Bacillus, Azotobacter, rat, and chicken (Ki or IC50 = 173, 500, 260, and 120 μM, respectively); also inhibits MDH1 (cytoplasmic) and MDH2 (mitochondrial) (IC50s = 1.3 and 0.065 mM, respectively)