The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidin- or avidin-dye reagents, such as(strept)avidin-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -beta-gal, etc. Sample types: cell cultures
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This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis.