ELISA, Western Blot. This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 265 kDa in size corresponding to Rif1 by western blotting in the appropriate cell lysate or extract.
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Description
This affinity purified antibody is directed against mouse Rif1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with Rif1 from human, chimpanzee, rat and dog as only a single amino acid residue change (92% homology) is found for the immunogen sequence. Cross reactivity with Rif1 homologues from other sources has not been determined. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 kDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 kDa) and mouse Rap1-interacting factor 1 (2426 267.1 kDa).
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids 2406-2419 of Mouse Rif1.