Titrated value sufficient to run approximately 10 mini blots.
Applications
ELISA, Immunohistochemistry, Western Blot. Anti-14-3-3 pS58 antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to the 14-3-3 proteins phosphorylated at Ser58 in the appropriate cell lysate or extract.
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Description
Anti-14-3-3 pS58 antibody is directed against rat 14-3-3 protein phosphorylated at S58. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated 14-3-3. Minimal reactivity occurs against non-phosphorylated 14-3-3. Reactivity against 14-3-3 occurs from rat and human sources. Reactivity is expected against the following species based on 100% sequence homology: bovine, canine, chicken, mouse, non-human primates, sheep, Xenopus and zebra fish.
Immunogen
Anti-14-3-3 pS58 antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58.
Other Names
14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1