ELISA, Immunohistochemistry, Western Blot. This antibody has been tested for use in ELISA and western blot. A 1:500 dilution was effective for staining FFPE human kidney tissue by Immunohistochemistry (IHC). Specific conditions for reactivity should be optimized by the end user. For western blot experiments, one can expect a band of ?110 kDa in size corresponding to active Notch 2 in the appropriate cell lysate.
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Description
This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues 2396-2409 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.
Gupta S, Li S, Abedin Md J, Wang L, Schneider E, Najafian B, Rosenberg M. Effect of Notch activation on the regenerative response toacute renal failure. Am J Physiol Renal Physiol 298: F209-F215, 2010. Published online before print October 14, 2009, doi: 10.?1152/?ajprenal.?00451.?2009