ELISA, Immunohistochemistry, Western Blot. Anti-Mouse IgG F(c) Antibody has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for Anti-Mouse IgG F(c) Antibody.
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Description
Anti-Mouse IgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
Immunogen
Anti-mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat.