ELISA, Immunohistochemistry, Western Blot. Anti-Mouse IgG F(c) antibody has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
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Description
Anti-Mouse IgG F(c) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
Immunogen
Anti-Mouse IgG was produced by repeated immunization with mouse IgG F(c) fragment in goat.
Other Names
Mouse IgG F(c) Antibody, Gt-a-Mouse F(c), Mouse F(c) in goat, Goat F(c) Secondary Antibody, goat anti-mouse IgG F(c) fragment antibody