KEY FEATURES: ? Sulfo-NHS-SS-Biotin is amine-reactive and ? Has a long chain spacer arm (24.3 A) separating the biotin group from the amino-reactive sulfo-NHS ester ? Water-soluble; can be used under physiologic conditions ? It is possible to cleave the biotin group from the cross-linked target protein under reducing conditions, such as in the presence of BME, DTT or TCEP APPLICATIONS: ? Sulfo-NHS-SS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein purification, detection or immobilization ? Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent. MW: 606.69. Purity ≥80% by NMR