Pyruvate, phosphate dikinase, PPDK, (E.C. 2.7.9.1) is found in microorganisms and plants where it catalyzes reversible interconversion of AMP, PPi and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate. PPDK plays an important role in plant photosynthesis and glycolytic pathway of endobiotic protozoa and bacteria where pyruvate, phosphate dikinase (PK) is absent; it functions in the process of ATP biosynthesis. In plants, PPDK is a pivotal regulator of the C4 photosynthetic cycle and the key limiting factor for maintaining photosynthesis at low temperature. It catalyzes the regeneration of PEP as the primary acceptor of atmospheric CO2 from pyruvate, ATP and phosphate. In addition, PPDK is also present in C3 leaves where it con
Size
100 assays
Concentration
n/a
Applications
? Measurement of PPDK activity in tissue/cell lysates and protein preparations ? Screening for specific inhibitors of PPDK activity that may affect microorganisms/plant biochemical pathways? Study of pyrophosphate-mediated glycolysis in bacterial and protozoan cell lysates? Detection of activity and light activation kinetics of PPDK from crude leaf extracts and recombinant protein? Study of photosynthesis in plants