A His-tag is an amino acid motif that contains at least six consecutive histidine (His) residues, often at the N- or C-terminus of a protein. More than 50% of recombinant proteins expressed by eukaryotic or prokaryotic expression systems are tagged with His-tag. Selective, sensitive and quantitative detection of protein levels and protein-protein binding partners expressed with His-tag is therefore a valuable tool in Life Science research. BioVision's His-Tag Protein ELISA Kit is a competitive-based ELISA that is easier, faster and more sensitive method to detect His-tag proteins expressed in both bacterial, insect and mammalian cells. This detection kit offers ready-to-use reagents, and can detect as low as 20 ng/ml of His-tag protein with
Size
100 assays
Concentration
n/a
Applications
This ELISA kit is used for quantitative measurement of His-Tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies