Periostin, BioAssay? ELISA Kit (OSF-2, Osteoblast Specific Factor 2) (Mouse)
Description
The mouse Periostin BioAssay? ELISA kit is to be used for the in vitro quantitative determination of mouse periostin in cell culture supernatants, serum and plasma. Sample Type: Cell culture supernatants, serum and plasma Intended Use: Quantitative ELISA Sensitivity: 10pg/ml Range: 31.25-2000pg/ml Specificity: Recognizes natural and recombinant mouse periostin. It has been tested on mouse periostin isoform 5 (should also detect mouse isoforms 1, 2 and 3 according to sequences). Mouse isoform 4 has not been tested. Test Principle: A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a