Protein S100-A12 (S100A12) BioAssay? ELISA Kit (Human)
Description
Protein S100-A12 (S100A12) BioAssay? ELISA Kit is a quantitative sandwich assay for the detection of S100A12 in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples. Detection Range: 15.6-1000pg/ml Sensitivity: <9.375pg/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Assay Principle: This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to S100A12. Standards and samples are added to the appropriate wells, then incubated. Aspirate and wash the plate. The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain S100A12, the antibody (Biotin) and Streptavidin (HRP) will exhibit a c