Enzyme-linked immunosorbent assay (ELISA) is the most commonly used method for detecting or identifying substances such as peptides, proteins, antibodies and hormones. The sensitivity of ELISA depends on the detection limits, colorimetric detection limits the assay sensitivity for the colorimetric substrates used. Chemiluminescent detection is an alternative variation of the standard enzyme colorimetric detection, characterized by its high sensitivity, broader dynamic range, and high signal-to-noise ratio. In chemiluminescence detection, horse radish peroxidase (HRP) substrates have shown improved sensitivity over colorimetric substrates. BioVision's SuperBrite? ELISA HRP Chemiluminescence substrate provides excellent sensitivity and