Identification of clones that contain the desired DNA insert is an essential step in molecular cloning procedure. Traditional methods for identifying the correct DNA inserts require growing up individual colonies, isolating plasmid DNAs, and then digesting with restriction enzymes to analyze each individual clone. Such traditional procedures are very time consuming, laborious, and expensive. The new InsertFinderTM PCR Quick Screening Kit is designed to quickly screen clone candidates using PCR technology. The procedure allows screen of colonies directly from plate, without growing up the cultures. The proprietary Cell Lysis Buffer provided with the kit can efficiently lyse cells (bacteria or yeast) without interfering with PCR reactions. Th