ELISA: 0.5-1.0 μg/mL, Immunoprecipitation: 1.0-2.0 μg/mL, Western Blot: 1 μg/mL
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Description
Mu-Calpain Monoclonal Antibody (15C10). The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I ) are composed of an 80 kD subunit and a 30 kD subunit. Wheras the 30 kD subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. Whereas m-clapain requires millimolar (mM) levels of calcium, μ-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- an μ- calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas