Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Dot Blot. This antibody is reactive with Notch 1 by western blotting. A strong response was also detected by ELISA against the immunizing peptide.This product was assayed by immunoblot against recombinant mouse Notch constructs in 293 cells. An 80 kDa band corresponding - Notch 1 was observed at a 1:500 dilution. This product was also assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG [Goat] and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 - 1:60,000 is suggested for this product.
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Description
The Notch-1 Antibody from Novus Biologicals is a rabbit polyclonal antibody to Notch-1. This antibody reacts with human, mouse, rat. The Notch-1 Antibody has been validated for the following applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Dot Blot.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch-1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Other Names
EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1