SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins.10X SDS-PAGE Running Buffer is suitable for laboratory involved in protein biochemistry. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
Size
1 L
Concentration
10X
Applications
This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required. 10X SDS-PAGE Running Buffer consists of 0.25 M Tris HCl, 1.92 M Glycine and 1% (w/v) Sodium Dodecyl Sulfate (SDS); pH 8.3. Meticulously prepared using ultra-pure reagents dissolved in highly polished pharmaceutical grade deionized water. Some yellow coloration of the 10X buffer may be observed. A slight coloration does not interfere with the performance of the running buffer.
Other Names
Sodium dodecyl sulfate polyacrylamide running buffer, Protein Gel Running Buffer, polyacrylamide gel running buffer.