A steroid-based cationic lipid that can be used to facilitate gene transfection; forms a complex with plasmid DNA (pDNA) and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher; facilitates transfection of the luciferase gene into H1299 cells, which can be inhibited by methyl-β-cyclodextrin and genistein; induces cytotoxicity in H1299 cells (IC50 = 92.7 μg/ml)