Tumor protein p53 (TP53, p53) is a widely conserved transcription factor and tumor suppressor commonly referred to as the ``guardian of the genome''. Due to the presence of transcriptional activation, DNA binding, and oligomerization domains, p53 responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in p53 gene are associated with numerous human cancers and about 50% of those mutations result in loss of function. Multiple genotoxic and non-genotoxic stimuli lead to stabilization and activation of p53 that are mediated by numerous post-translational modifications. The N-terminus of p53 is heavily phosphorylated, wh
Size
50 assays
Concentration
n/a
Applications
Detection of nuclear translocation of phosphorylated p53 in mammalian cells