The Quantikine Human MIP-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MIP-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human MIP-1 alpha. Range: 31.20 - 1000 pg/mL (Cell Culture Supernates), 46.90 - 1500 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma). Sensitivity: 10 pg/mL. Time: 3.5 hours or 4.5 hours