A highly selective non-ATP competitive allosteric Akt inhibitor with IC50's of 8 nM, 2 nM and 65 nM for Akt1, Akt2 and Akt3, respectively. MK-2206 potently inhibits phosphorylation of Thr308 and Ser473 in 3T3-L1 adipocytes with IC50 values of 0.11 and 0.18 μM, respectively as well as downstream effects of insulin on GLUT4 translocation (IC50 = 0.47 μM) and glucose transport (IC50 = 0.14 μM). In addition, treatment with MK-2206 causes a robust, concentration-dependent activation of autophagy in human glioma cell lines LN229 and T98G. MW: 480.39. Purity ≥98% by HPLC