ELISA, Western Blot. This product was assayed by immunoblot and found to be reactive against NF-Y (B subunit specific) at a dilution of 1:2,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y. Minimal reactivity was observed against the A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against NF-Y using 0.5 to 1.0 μl per assay.
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Description
This product was prepared from monospecific antiserum by delipidation and defibrination.
Immunogen
NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Other Names
rabbit anti-NF-Y Antibody, Nuclear transcription factor Y subunit beta, Nuclear transcription factor Y subunit B, CAAT box DNA-binding protein subunit B, NFYB