ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-Histone H3 [Trimethyl Lys27] antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract. Epi-Plus? antibody production in collaboration with Novus Biologicals.
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Description
Anti-Histone H3 [Trimethyl Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Lys27 me3] from other sources has not been determined.
Immunogen
Histone H3 [Trimethyl Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27me3 site of human Histone H3.