ELISA, Immunohistochemistry, Western Blot. This antibody reacts with human APC2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC11) does occur. A 93.8 kDa band corresponding to human APC2 is detected. Most cell lines or tissues expressing APC2 can be used as a positive control. Researchers should determine optimal titers for other applications.
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Description
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human APC2. Cross reactivity may also occur with APC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 810-822 of Human APC2 (C-terminal) coupled to KLH.
Yu H,Peters JM,King RW,Page AM,Hieter P,Kirschner MW, (1998) Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science 279:5354, 1219-1222.Zachariae W,Shevchenko A,Andrews PD,Ciosk R,Galova M,Stark MJ,Mann M,Nasmyth K, (1998) Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279:5354, 1216-1219.