ELISA, Western Blot. This purified polyclonal antibody reacts with yeast APG8 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 14 kDa band corresponding to yeast APG8 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
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Description
Anti-ATG8 antibody is also known as Anti-APG8 and is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG8 protein.
Other Names
rabbit anti-APG8 Antibody, APG-8, APG 8, ATG-8, ATG 8, anti-ATG8 Antibody, Autophagy-related protein 8, Autophagy-related ubiquitin-like modifier ATG8, Cytoplasm to vacuole targeting protein 5, AUT7, CVT5