Step 6. Subcloning - Cells from up to 5 strongly positive fusion wells are expanded, isotyped and subcloned by limiting dilution. More subclones can be processed at your request for an additional charge (see next page).Step 7. Screening, Expansion and Cryopreservation - Subclones are screened by ELISA, positives expanded, and screened again. Up to two (2) positive subclones from each parental subclone will be expanded, frozen and stored for up to six (6) months.