Phosphatidylethanolamine (PE), also known as cephalin is the second most abundant phospholipid in animal and plant tissues, and is present on the cytoplasmic side of the plasma membrane. Phosphatidylethanolamine is a neutral phospholipid consisting of a phosphatidyl group ester linked to an ethanolamine molecule. Its functions include membrane fission/fusion, maintenance of membrane curvature and stabilization of membrane proteins, since it can form hydrogen bonds with proteins through an ionizable amine group. It acts as a chaperone during assembly of membrane proteins and aids in their translocation from the cytoplasm to the membrane. It is also involved in secretion of very low density lipoproteins in the liver. The Phosphatidylethanolamine assay kit is a microplate based enzymatic assay for the quantitation of PE in cells and tissues. PE Converter hydrolyses PE to an intermediate, which converts a colorless probe to a fluorescent product via enzymatic reaction (Ex/Em: 535/587). The intermediate formed through PE converter hydrolysis is specific to phosphatidylethanolamine. Thus no other phospholipids (i.e. phosphatidylcholine, phosphatidylinositol or phosphatidic acid) will be detected, making the kit highly specific. This assay kit can detect as low as 0.2 nmol per well. Sample types: cell lipid extract, tissue lipid extract
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Applications
This assay provides a convenient and non-ELISA alternative to detect Phosphatidylethanolamine amount in biological samples.