Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. Lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 56 nm, is proportionate to the lactate concentration in the sample. Applications: Direct Assays: Lactate in serum, plasma, and cell media samples. Key Features: Sensitive and accurate. Detection limit: 96-well plate assay: 0.05mM and linearity up to 2mM L-Lactate. For cell culture samples containing phenol red: 0.1mM and linearity up to 1mM L-Lactate in 96-well plate assay. Convenient. The procedure involves adding a single working r